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农杆菌介导禾本科牧草遗传转化的研究进展
张月1, 李莹1, 戴绍军2,3
1.东北林业大学 生命科学学院 东北盐碱植被恢复与重建教育部重点实验室, 黑龙江 哈尔滨 150040;2.上海师范大学 生命科学学院, 上海 200234;3.上海师范大学 上海植物种质资源工程技术研究中心, 上海 200234
摘要:
建立禾本科牧草的遗传转化体系对于种质资源利用具有重要意义.近年来,假俭草(Eremochloa ophiuroides)、黑麦草(Lolium perenne)、结缕草(Zoysia japonica)、柳枝稷(Panicum virgatum)、高羊茅(Festuca elata)、二穗短柄草(Brachypodium distachyum)、匍匐剪股颖(Agrostis stolonifera)、朝鲜碱茅(Puccinellia chinampoensis)、羊草(Leymus chinensis)、金发草(Pogonatherum paniceum)、双花草(Dichanthium annulatum)等多种禾本科牧草遗传转化的转化方法不断得到优化与完善.该文总结了近年来禾本科牧草在遗传转化体系优化的研究进展,探讨了植物受体材料、转化条件、共培养时间、乙酰丁香酮浓度、抑菌剂浓度、筛选剂选择压等因素对转化效率的影响.
关键词:  禾本科牧草  遗传转化  影响因素
DOI:10.3969/J.ISSN.1000-5137.2021.01.004
分类号:Q939.9
基金项目:黑龙江省自然科学基金重点项目(JJ2019ZZ0235);上海市科委科研计划(17391900600)
Agrobacterium-mediated genetic transformation of gramineous forages
ZHANG Yue1, LI Ying1, DAI Shaojun2,3
1.Key Laboratory of Northeast Saline-Alkali Vegetation Restoration and Reconstruction, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin 150040, Heilongjiang, China;2.College of Life Sciences, Shanghai Normal University, Shanghai 200234, China;3.Development Center of Plant Germplasm Resources, Shanghai Normal University, Shanghai 200234, China
Abstract:
The establishment of a genetic transformation system for gramineous forages is of great significance to the utilization of germplasm resources. In recent years, the genetic transformation methods of various gramineous forages such as Eremochloa ophiuroides, Lolium perenne, Zoysia japonica, Panicum virgatum, Festuca elata, Brachypodium distachyum, Agrostis stolonifera, Puccinellia chinampoensis,Leymus chinensis,Pogonatherum paniceum and Dichanthium annulatum have been continuously optimized. This article summarized the recent research progress in the optimization of the genetic transformation systems of gramineous forages,and discussed the effects of plant receptor material,transformation condition,co-cultivation time, acetosyringone concentration,bacteriostatic agent concentration,selection pressure of screening agents and other factors on the transformation efficiency.
Key words:  gramineous forage  genetic transformation  effect factors