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水稻低温敏感叶色突变体tcd32鉴定与遗传分析
王妍斌1, 周优1, 林冬枝2, 董彦君2
1.上海师范大学生命与环境科学学院;2.上海师范大学生命与环境科学学院,上海师范大学遗传研究所
摘要:
本研究对粳稻“嘉花1号”经60Coγ诱变处理获得的稳定遗传低温敏感叶色突变体tcd32进行了表型鉴定与遗传分析。在20℃条件下,该突变体表现为白色,光合色素含量明显下降,叶绿体发育不完整,植株最终枯萎死亡;在25℃条件下,三叶期之前幼苗表现为黄色,从第4叶开始逐渐转为黄绿色,光合色素含量也明显下;而在30℃条件下,其表型与野生型相比没有明显差异。通过对培矮64S与tcd32杂交的F2代分离群体进行遗传分析,结果表明该低温敏感叶色突变体性状是受一对隐性核基因(tcd32)控制,利用图位克隆技术将tcd32基因定位在水稻第3染色体上顶端537kb区域内,发现 tcd32是一个新的水稻早期叶绿体发育相关基因,为后续的研究奠定了基础。
关键词:  水稻:叶绿体:温度敏感:叶色突变  基因定位
DOI:
分类号:Q344
基金项目:国家重点研发项目(2016YFD0100902)、上海市科委项目(16ZR14253000、1639190070010)和上海市教委项目(2017-01-07-00-02-E00039)。
Identification and genetic analysis of low temperature sensitive leaf color mutant tcd32 in rice
wangyanbin1, zhouyou1, lindongzhi1,2, donyanjun1,2
1.College of life and Environmental Sciences, Shanghai Normal University;2.Institute of genetic research, Shanghai Normal University
Abstract:
In this study, the identification and genetic analysis of a stable genetic low temperature sensitive leaf color mutant tcd32 obtained by 60Co gamma mutagenesis in the japonica rice "Jiahua 1" were carried out. Under the condition of 20℃, the tcd32 mutant showed white leaves and obviously low level of photosynthetic pigments, have not intact chloroplast, eventually mutant plants withered to death; under the condition of 25℃, the 3-leaf seedlings were yellow, then began to turn yellowish green from the fourth leaves, also showed lower level of photosynthetic pigments. However, at 30 ℃, the tcd32 mutant had no obvious difference from the wild type phenotype. Based on genetic analysis of F2 segregating population form the cross of Peiai 64S and tcd32, it was shown that the low-temperature sensitive mutant trait was controlled by a single recessive nuclear gene (tcd32). Then through map-based cloning, the tcd32 gene was located to the 537kb region at the top of chromosome 3 in rice, which is a novel gene associated with to early chloroplast development in rice. The obtained results laid a foundation for the further study .
Key words:  Rice: chloroplast: temperature sensitive: leaf color mutation  gene location